Molecular diagnosis of the Demodex folliculorum parasite by 18SrRNA gene in Iraq

نویسندگان

چکیده

Demodex folliculorum It is one of the most types microscopic external parasites that infect humans, which feeds on fat skin, around hair follicles and causes many diseases, including Perioral dermatitis, Demodectic Blepharitis, Pityriasis folliculorum, Rosacea leads to induction allergic inflammatory reactions against its antigens.The current study aimed detect parasite in humans by using PCR technique primers (18S rRNA) genes responsible for diagnosing parasite. Where DNA was extracted from samples collected those infected with parasite, were performed a machine clone DNA. Then, nucleotide sequence gene studied, path length products 802 base pairs, they sent NCBI website, all generated sequences recorded GenBank database (entry number: MW186179.1, MW186180.1), A phylogenetic tree established Neighbor joining method MEGA X version two isolates human links genetically compared NCBI-BLAST respect D. isolate Iraq. 18SrRNA used confirmed genetic identification. Iraqi are 100% identical Hungary KC894848.1), but less similar other Spain, China, Thailand Am905316.1, HQ7280000.1, KY305348.1) respectively

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ژورنال

عنوان ژورنال: Nucleation and Atmospheric Aerosols

سال: 2023

ISSN: ['0094-243X', '1551-7616', '1935-0465']

DOI: https://doi.org/10.1063/5.0136716